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KMID : 0352720070310030154
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Journal of Ginseng Research
2007 Volume.31 No. 3 p.154 ~ p.158
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Effects of Various Chelating Agents on Accumulation of Germanium in Ginseng Adventitious Roots in Submerged Culture
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Chang Eun-Jung
Oh Hoon-Il
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Abstract
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In order to increase the content of germanium in ginseng adventitious roots, the effects of chelating agents on germanium content and root growth were investigated in the submerged cultures of ginseng adventitious roots. Chelating agents such as citric acid, oxalic acid, phosphoric acid, EDTA (Ethylenediamine tetraacetic acid) or EGTA (Ethylene glycol-bis (β-aminoethylether)-tetraacetic acid) were administrated in the submerged culture of ginseng root containing 50 ppm GeO₂. After 6 weeks of cultivation, fresh weight, germanium and saponin contents in the roots were analyzed. Among chelating agents, addition of 1.0mM phosphoric acid was found to be best for germanium accumulation. Under this condition, germanium content increased 1.4 times as compared to that of the control. The germanium content in the adventitious roots also increased with addition of EDTA or EGTA, while they inhibited the growth of ginseng adventitious root. Citric and oxalic acids were not effective for increasing germanium content in adventitious roots. As the results, it suggests that the phosphoric acid can be proved as the optimal agent for the enhancement of germanium accumulation in ginseng adventitious roots. These results can be served as a guideline for the mass production of ginseng adventitious roots containing germanium by large-scale production
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KEYWORD
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Ginseng, adventitious root, germanium, chelating agent
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